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      产品选择指南

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      当前位置:首页 > 转染试剂 > Xfect Single Shots

          好的转染实验(Transfection reagent)就像是一件精致的艺术品,是转染效率(transfection efficiency)和细胞活性(cell viability)完美的平衡。Xfect转染系列是Clontech公司为细胞学科学家精心奉献的一只画笔,助您创作出更美好的画作!

      高转染效率

        ■ 常用细胞系
        Xfect transfects the following 5 most commonly transfected cell lines with very high efficiency:
       
      Cell Type Transfection Efficiency
      293T 97%
      HEK-293 93%
      HeLa 85%
      NIH/3T3 82%
      CHO 83%
        ■ 难转染细胞系
        Xfect also outperforms popular competitor transfection reagents in Jurkat cells, which are notoriously difficult to transfect. Xfect Transfection Reagent produced >40-fold higher transfection efficiency than Product L. This transfection reagent can also be used to transfect human adult stem cells. We used Xfect to transfect human adipose-derived stem cells (hADSCs) with a transfection efficiency of 92%.

      高细胞活性(very high cell viability)

        Cells Transfected with Xfect Have High Viability  
       
        TXfect(%) Product(%)
      HeLa 79.4%±17.9 53.2±26.7
      HEK-293 63.5±12.5 52.3±12.4
      CHO-K1 86±0.8 90.9±10.7
      MCF7 51.4±10.6 29.7±21.3
       

      方便使用(ease of use)

        ■ 专门准备了在血清(in presence of serum)存在条件下的简易操作方案
        ■ 优化的“傻瓜相机式操作”试剂盒包装
        ■ 专门开发了miRNA/siRNA/mRNA以及protein转染专用的产品

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